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Panax stipuleanatus H. T. Tsai et K. M. Feng Extracts Induced the Relaxation of Isolated Smooth Muscles and Elevated eNOS phosphorylation
Vu Thi Thom, Ho Thi Thu Ha, Dinh Doan Long, Duong Thi Ly Huong

Abstract
This study aimed to explore the effect of Panax stipuleanatus H. Tsai et K.M. Feng on 3 types of isolated smooth muscles: trachea, bladder, and corpus cavernosum. Five extracts of P. stipuleanatus including total extract (PST), n-butanol extract (PSBt), n-hexan extract (PSnH), dichloromethane extract (PSDcM), and saponin riched extract were used for smooth muscle relaxation and eNOS phosphorylation tests. The results showed that the total extract (PST) and the n-butanol extract of P. stipuleanatus (PSBt) induced the relaxation of trachea, bladder, and corpus cavernosum while PSnH and PSDcM did not show similar effect. Moreover, performing experiment for eNOS phosphorylation in human umbilical vein endothelial cells (HUVEC) we showed that the PST and saponin riched extract elevated the nitric oxide production (NO) as well as the eNOS phosphorylation expression.

Keywords: Panax stipuleanatus, bladder, corpus cavernosum, isolated smooth muscles, trachea, eNOS phosphorylation