Phytochemical and Thin Layer Chromatography Analyses of Lophopetalum wallichii Kurz Barks Native to Cambodia
Sereynich Va, Pichmony Orm, Sreynich Lean, Seaklim Vorn, Meyli Phuy, Huykhim Ung, Sin Chea, Samell Keo
Abstract
Lophopetalum wallichii Kurz native to Cambodia has been widely used for healing various ailments such as asthma, urinary disorders, influenza, arthritis, malaria, and cancers. This study aimed at identifying the phytochemical components of ethanolic extracts of the Lophopetalum wallichii Kurz barks, native to Cambodia, and at profiling its Thin Layer Chromatography (TLC) fingerprints. The dried barks of Lophopetalum wallichii Kurz were subjected to the Ultrasound-Assisted Extraction (UAE) with ethanol; the extracts were subsequently preserved for the analyses of phytochemicals and TLC. The phytochemical screening of Lophopetalum wallichii Kurz revealed positive tests of alkaloids, phenolic compounds, tannins, flavonoids, terpenoids, cardiac glycosides, saponins and resins. TLC was developed with a mobile phase system Chloroform:Ethanol (1:1) and investigated under 254-366 nm UV light and 10%-H2SO4, and provided a clear separation with different Rf values. These findings together could be used for the isolation of bioactive pure compounds.
Keywords: Lophopetalum wallichii; Thin layer chromatography
Sereynich Va, Pichmony Orm, Sreynich Lean, Seaklim Vorn, Meyli Phuy, Huykhim Ung, Sin Chea, Samell Keo
Abstract
Lophopetalum wallichii Kurz native to Cambodia has been widely used for healing various ailments such as asthma, urinary disorders, influenza, arthritis, malaria, and cancers. This study aimed at identifying the phytochemical components of ethanolic extracts of the Lophopetalum wallichii Kurz barks, native to Cambodia, and at profiling its Thin Layer Chromatography (TLC) fingerprints. The dried barks of Lophopetalum wallichii Kurz were subjected to the Ultrasound-Assisted Extraction (UAE) with ethanol; the extracts were subsequently preserved for the analyses of phytochemicals and TLC. The phytochemical screening of Lophopetalum wallichii Kurz revealed positive tests of alkaloids, phenolic compounds, tannins, flavonoids, terpenoids, cardiac glycosides, saponins and resins. TLC was developed with a mobile phase system Chloroform:Ethanol (1:1) and investigated under 254-366 nm UV light and 10%-H2SO4, and provided a clear separation with different Rf values. These findings together could be used for the isolation of bioactive pure compounds.
Keywords: Lophopetalum wallichii; Thin layer chromatography
