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In vitro anti-coagulation activity of Panax bipinnatifidus Seem. and Panax stipuleanatus H.T. Tsai et K. M. Feng saponin enriched extracts
Vu Thi Thom, Nguyen Thi Thu Giang, Nguyen Hoang Ngan, Dang Van Diep, Duong Thi Ly Huong

Abstract
Panax bipinnatifidus Seem. and Panax stipuleanatus H.T. Tsai et K.M. Feng are rich saponin containing medicinal plants primarily grown in the North-West Moutain region in Vietnam. The present study aims to explore the anticoagulant activity of saponin enriched extracts isolated from these herbal plants using prothrombin time (PT) and activated partial thrombin time (aPTT). Heparin (0.1 IU/mL) was used as positive control, while DMSO (0.04 %) and NaCl (0.9 %) were used as negative and solvent control. Heparin showed clearly effect on aPTT prolongation the time up to 58.14 seconds while those in DMSO and NaCl group were 34.86 and 34.63 seconds, respectively. In contrast to our previous study using crude extracts, the saponin-enriched extracts of Panax bipinnatifidus Seem. and Panax stipuleanatus H.T. Tsai et K.M. Feng did not express any anticoagulant activity (p>0.05). In conclusion, our study demonstated that the anticoagulant activity of crude extracts of Panax bipinnatifidus Seem. and Panax stipuleanatus H.T. Tsai et K.M. Feng was not caused by saponin compound.

Keywords: Anti-coagulant activity, Panax bipinnatifidus Seem., Panax stipuleanatus H.T. Tsai et K. M. Feng, saponin enriched extract