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Secondary metabolites from the leave extracts of Morus alba L.
Tofael Ahmed, Mohammad Shoeb*, Md. Nazrul Islam, Md. Mizanur Rhaman, Elias Ahmed, Nilufar Nahar

Abstract
Morus alba L., a commercially important plant has medicinal value and grows naturally in Bangladesh. The leaves of M. alba were extracted with aqueous 80% ethanol and was evaporated into dryness. The dry mass was suspended in water and was successively partitioned with dichloromethane, ethyl acetate, and n-butanol. By repeated normal phase silica gel column chromatography of dichloromethane and ethyl acetate extracts afforded four secondary metabolites namely, octadecanol, 4-hydroxy octadec-6,9-dienoic acid, β-sitosterol, and stigmasterol. The structures of all metabolites were confirmed by 1H- and 13C-NMR spectroscopic studies. Analysis of fatty acid compositions of an oily fraction obtained from a column of DCM extract by gas chromatography equipped with a flame ionization detector (GC-FID) revealed that palmitic acid was predominant acid in the oil.

Keywords: Morus alba, fatty acids, Bangladesh, flame ionization detector