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Antibacterial, Antioxidant, Anti-inflammatory, and Anti-acethylcholinesterase Activity of Mikania scandens (L.) Willd (Climbing Hempvine)
Sumi Wijaya, Ting Kang Nee, Khoo Teng Jin, Christophe Wiart

Abstract
Abstract: Mikania scandens, known as Climbing hempvine has been used in many countries as traditional medicines. In this study, hexane, ethyl acetate and ethanol extracts from Mikania scandens (L.) Willd. tested its anti-cholinesterase, antioxidant, anti-inflammatory, and antibacterial activity. Anti-inflammatory activity is conducted by the 5-lipoxygenase inhibition activity, while the activity of anti-cholinesterase is determined by the TLC-bioautography test. Antioxidant activity was measured using FRAP, 2,2-diphenyl-1- picrylhydrazyl (DPPH) and bleaching test of β-carotene methods. Antibacterial activity was evaluated with pour plate diffusion test, death kinetic assay, minimum bactericidal concentration, and minimum inhibitory concentration. The ethanol extract showed potential as antioxidant and anti-inflammatory activity. The hexane extract showed the highest bacterial growth inhibition, meanwhile hexane and ethanol extracts generate the best acetylcholinesterase inhibitory activity. The total value of the phenolic content was measured using Folin-Ciocalteu method and the value is in the range of 106.67 mg/g to 1066.67 mg/g equivalent gallic acid. The results show that Mikania scandens (L.) Willd. can provide a natural source of anti-cholinesterase, antioxidant, anti-inflammatory, and antibacterial agents.

Keywords: antibacterial; antioxidant; anti-inflammatory; anti-cholinesterase; Mikania scandens; phenolics