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Studies of Marine Seaweed Sargassum flavicans
Forkan Saroar, Mohammad Shoeb, Md. Nazrul Islam, Md. Mizanur Rhaman, Rafiza Islam, Nargis Parvin

Abstract
The marine seaweed Sargassum flavicans belonging to the class Phaeophyceae grows naturally in Saint Martin’s Island, Cox’s Bazar, Bangladesh. S. flavicans was collected from the Island for fatty acid analysis and investigation of its biological properties. The dried seaweed (~210 g) was extracted with a mixture of dichloromethane and methanol (1:1). The crude extract (9.0 g) was suspended in water and was partitioned successively with hexane, dichloromethane, and finally with 2-butanol. Cytotoxicity assay of different extracts of the seaweed was carried out on the HeLa cell line, a human cervical carcinoma cell line, and none of the extracts was found to be cytotoxic. Antioxidant activity, antioxidant capacity, and phenolic content of extracts were also evaluated and the dichloromethane extract contained more phenolic content (264.67 ± 0.87 mg gallic acid equivalent per gram of dry extract) and antioxidant capacity (388.0 ± 9.55 mg ascorbic acid equivalent per gram of dry extract) compared to other extracts of S. flavicans. Analysis of fatty acid compositions in oil was also carried out by gas chromatograph equipped with flame ionization detector (GC-FID) and palmitic acid (46.75 %), stearic acid (18.45 %) as well as oleic acid (19.93 %) were the major acids in the oils.

Keywords: Sargassum flavicans, palmitic acid, myristic acid, antioxidant activity, cytotoxicity.