Pharmacognostical, phytochemical and pharmacological potentials of Cannabis sativa L.
Sudipta Baroi, Achintya Saha, Ritesh Bachar, Sitesh C Bachar
Abstract
Cannabis sativa contains promising groups of cannabinoids with reported anticancer properties. For the evaluation of pharmacognostical, phytochemical and pharmacological potentials, the herb was collected from the Department of Narcotics Control, Government of Bangladesh. The plant material was extracted with hexane, ethylacetate, methanol and water separately using sohxlet apparatus. The macroscopic and microscopic examinations of the plant sample followed by phytochemical screenings of different solvent extracts showed the presence of flavonoids, phenols, sterols and terpenoids in major. The extracts were further studied for biological screening in brine shrimp lethality as a mean of anticancer activity using vincristine sulfate as positive standard. The LC50 of the hexane part (0.398±0.001 μg/ml) and ethyacetate extract (0.450±0.003 μg/ml) exhibited potential activity in comparison to vincristine sulfate (0.316±0.002 μg/ml). The LC50 values of hexane and ethyacetate extracts are indicative for anticancer activity, and the presence of terpinoids like compounds in those extracts are indicative of potential functionality of cannabinoids like compounds.
Keywords: Cannabis sativa, pharmacognostical, phytochemical, pharmacological potential, brine shrimp lethality, anticancer activity screening.
Sudipta Baroi, Achintya Saha, Ritesh Bachar, Sitesh C Bachar
Abstract
Cannabis sativa contains promising groups of cannabinoids with reported anticancer properties. For the evaluation of pharmacognostical, phytochemical and pharmacological potentials, the herb was collected from the Department of Narcotics Control, Government of Bangladesh. The plant material was extracted with hexane, ethylacetate, methanol and water separately using sohxlet apparatus. The macroscopic and microscopic examinations of the plant sample followed by phytochemical screenings of different solvent extracts showed the presence of flavonoids, phenols, sterols and terpenoids in major. The extracts were further studied for biological screening in brine shrimp lethality as a mean of anticancer activity using vincristine sulfate as positive standard. The LC50 of the hexane part (0.398±0.001 μg/ml) and ethyacetate extract (0.450±0.003 μg/ml) exhibited potential activity in comparison to vincristine sulfate (0.316±0.002 μg/ml). The LC50 values of hexane and ethyacetate extracts are indicative for anticancer activity, and the presence of terpinoids like compounds in those extracts are indicative of potential functionality of cannabinoids like compounds.
Keywords: Cannabis sativa, pharmacognostical, phytochemical, pharmacological potential, brine shrimp lethality, anticancer activity screening.
