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Phytochemical analysis and cytotoxicity evaluation of flowering buds of Bauhinia variegata
Anham Shahid Abbasi, Qazi Najam-us-Saqib, Nisar-ur-Rahman, Atta-ur-Rehman

Abstract
The current investigation was focused to access the phytochemical studies of the crude aqueous extract and methanol extract of flowering buds of Bauhinia variegata contains secondary metabolites like saponins, flavonoids, carbohydrates, proteins, amino acids, and terpenoids. The quantity of these secondary metabolites varied in crude aqueous extract and methanol extract of the buds. The highest levels of phenolics, flavonoids, and terpenoids were observed in methanol extract with the respective standard drugs. The amino acid content was highest followed by phenol and flavonoids.The quantitative determination of secondary metabolites proteins, amino acids, flavonoids, saponins, etc. were also conducted and found that crude aqueous extract of the drug contains the maximum amount of secondary metabolites. In cytotoxic studies (brine shrimp lethality assay) the use of the extract of methanol was reported as potent with LD50 of 241.778 μg/ml as compared to aqueous extract with LD50 of 489.706 1μg/ml.

Keywords: Bauhinia variegata, phytochemical analysis, cytotoxic activity, Secondary metabolites