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Phytochemical screening and biological studies of Cynodon dactylon (L.) Pers. extracts
Dikshita Lama, Dharma Prasad Khanal, Bishwo Raj Dhungana and Bibek Sigdel

Abstract
A traditionally well-known perennial grass Cynodon dactylon (L.) Pers. has a variety of medicinal use such as a folk remedy in treatment of many symptoms and diseases like cramps, measles, tumors, wounds, warts, fever, and rheumatic affections. The objective of the present study was to evaluate antiulcer activity of the aerial extracts of Cynodon dactylon (L.) Pers in experimentally induced ulcers in rats. Cynodon dactylon (L.) Pers grass was collected from the Dhankuta district, Nepal, washed with water to, shed dried for a few days, cut into small pieces, and again shed dried for 3 weeks. After that it was pulverized in a grinder. Extraction was carried out using Methanol. The qualitative and quantitative Phytochemical screening was carried out using standard protocols. GC-MS analysis of the extract was done. The reducing power activity of the extracts of Cynodon dactylon (L.) Pers. was determined by the slight modification of the method of Oyaizu, 1986 (Bhalodia et al., 2013). In-vivo antiulcer activity was carried out taking Wistar Albino Rats in indomethacin induced ulcer. The methanolic extract of grass powder of Cynodon dactylon (L.) Pers. showed the presence of alkaloids, flavonoids, and cardiac glycosides in phytochemical screening. Similarly, the GCMS analysis of methanolic extract of grass of Cynodon dactylon (L.) Pers. showed the presence of 6 compounds. The extract showed indicative amount of TPC and TFC. The antioxidant activity by RPA and DPPH was also indicative. Similarly, the methanolic extract of the grass of Cynodon dactylon (L.) Pers significantly (p<0.05) inhibited ulcer index, free acidity and total acidity in indomethacin induced ulcer models as compared to positive control group. The methanolic extract of grass of Cynodon dactylon (L.) Pers. can be used as a new source of antiulcer agent. The methanolic extract of grass of Cynodon dactylon (L.) showed the impressive ulcer protection in indomethacin induced ulcer in experimental rats, so it may be used for the search of novel antiulcer agents.

Keywords: Peptic ulcer, ranitidine, Cynodon dactylon (L.) Pers., ulcer index, indomethacin, methanolic-extract