Preparation of Cleome gynandra Linn. (Maman) water extract with high antioxidant properties
Mohd Kamal N.H., Chee B.J., Zaridah M.Z., Muhammad Danial C.R., Abd Rashid L., Azman M., Mohd Khair A., Hafizi R., Wahid, N.S.

Abstract
Cleome gynandra Linn. (Maman) is a common herb eaten and used as a medicinal plant by many people around the world. The main objective of this study was to determine the antioxidant activity in various parameters which are temperature (40oC, 60oC, 80oC, and 100oC) and time (30 min, 1hr, 2hrs, and 3hrs) for the preparation of C. gynandra water extract. Cleome gynandra powder was placed in a tube containing water and heated at different temperatures of 40oC, 60oC, 80oC, and 100oC. For each temperature, 
the extract was incubated at different intervals of 30 min, 1 h, 2 hrs, and 3 hrs. In this study, four tests such as DPPH, FRAP, TPC, and MDA assay are performed. The results showed that different parameters had different antioxidant activities. Based on the results, the parameter of 80oC for 30 min showed the highest 
antioxidant activity.

Keywords: Cleome gynandra Linn, antioxidant activity, DPPH, FRAP, TPC, MDA assay