Preparation of Legazpia polygonoides Water Extract with Different Parameters (Temperatures and Extraction Times): Antioxidant and Anti-lipid peroxidation
Mohd Kamal N.H., Zaridah M.Z., Azman M., Muhammad Danial C.R., Khairun Hazira K.H.
Abstract
Legazpia polygonoides is an herb belonging to the Linderniaceae family. This plant is traditionally used to treat eye infections and improve eyesight. However, scientific experiments using L. polygonoides are still scarce. This study aimed to determine the level of antioxidants and their ability to prevent lipid peroxidation. The dried L. polygonoides powder was placed in a tube of water and heated to different temperatures of 40oC, 60oC, 80oC and 100oC. For each temperature, the extracts were incubated at different times, e.g. 30 minutes, 1 hour, 2 hours, and 3 hours. The results of the DPPH test showed that the water extract of L. polygonoides prepared with the parameter 60oC / 3 hours had the highest inhibition of DPPH compared to the other parameters. Furthermore, the FRAP test showed that the aqueous extract of L. polygonoides prepared with the parameter 60oC/3 h had the highest FRAP value compared to the other parameters. For the TPC test, the preparation of aqueous extract of L. polygonoides with the parameter 100oC / 30 min had the highest phenol content compared to the other parameters. For the MDA test, the test results showed that the extracts produced using the 100oC/3 h parameter had lower levels of malondialdehyde compared to the other parameters. This study shows that L. polygonoides has the potential to be used as a source of antioxidants. It can be used to prevent lipid peroxidation.
Keywords: L. polygonoides, temperature, duration time, antioxidant, toxicity
Mohd Kamal N.H., Zaridah M.Z., Azman M., Muhammad Danial C.R., Khairun Hazira K.H.
Abstract
Legazpia polygonoides is an herb belonging to the Linderniaceae family. This plant is traditionally used to treat eye infections and improve eyesight. However, scientific experiments using L. polygonoides are still scarce. This study aimed to determine the level of antioxidants and their ability to prevent lipid peroxidation. The dried L. polygonoides powder was placed in a tube of water and heated to different temperatures of 40oC, 60oC, 80oC and 100oC. For each temperature, the extracts were incubated at different times, e.g. 30 minutes, 1 hour, 2 hours, and 3 hours. The results of the DPPH test showed that the water extract of L. polygonoides prepared with the parameter 60oC / 3 hours had the highest inhibition of DPPH compared to the other parameters. Furthermore, the FRAP test showed that the aqueous extract of L. polygonoides prepared with the parameter 60oC/3 h had the highest FRAP value compared to the other parameters. For the TPC test, the preparation of aqueous extract of L. polygonoides with the parameter 100oC / 30 min had the highest phenol content compared to the other parameters. For the MDA test, the test results showed that the extracts produced using the 100oC/3 h parameter had lower levels of malondialdehyde compared to the other parameters. This study shows that L. polygonoides has the potential to be used as a source of antioxidants. It can be used to prevent lipid peroxidation.
Keywords: L. polygonoides, temperature, duration time, antioxidant, toxicity
