Toxicity study of Kyllinga brevifolia and Scurrula parasitica using brine shrimp lethality test
Mohd Kamal N.H., Ihsan Safwan K., Zaridah M.Z., Susilatul Ziana A., Azman M., Muhammad Syahmi A., Ahmad Tirmizi A., Aida Adrieanna B.H., Alia Shafika B., Nur Khairah A.K.

Kyllinga brevifolia is an herb whose rhizome is used in traditional medicine as a refreshing drink and is said to have digestive, diuretic, sedative, tonic, antispasmodic, and diaphoretic properties. Scurrula parasitica has neuroprotective, sedative, anticancer, immunomodulatory, antiviral, diuretic, antihypertensive, antioxidant, antimutagenic, antiviral, antihepatotoxic, and antiviral effects. The use of these two herbs in health products needs to be studied more carefully as the demand for herbal products is increasing nowadays. Thus, the main purpose of this study is to determine the toxicity of the aqueous extract of K. brevifolia and S. parasitica using the brine shrimp lethality test (BSLT). The leaves of the two plants were dried in an oven at a temperature of 55°C for 48 hours. The dried leaves were ground and boiled at 100oC for 1 hour. BSLT test was performed with 8 replicates at 100, 50, 25, 12.5, 6.25, 3.125, 1.562 0.781, 0.39, 0.195, and 0.097 mg/mL. K. brevifolia was not significantly different from the untreated controls and had no lethal concentration 50 (LC50). In contrast, the LC50 of S. parasitica was 50 mg/mL.

Keywords: Kyllinga brevifolia, Scurrula parasitica, brine shrimp lethality test, Artemia salina, water extract