Antioxidant effect of aqueous Achyranthes aspera L. extract on hydrogen peroxide (H2O2)-induced zebrafish Model
Mohd Kamal NH, Ihsan Safwan K, Zaridah MZ, Muhammad Danial CR, Azman M, Siti 
Atiqah MA

Oxidative stress (OS) is caused by free radicals such as reactive oxygen species (ROS) in the human body. An excessive quantity of hydrogen peroxide (H2O2) would increase the levels of ROS, resulting in lipid peroxidation (LPO) and oxidative stress that can lead to further oxidative damage. Extract of Achyranthes aspera L. possesses well-documented antioxidant properties. The objective of the study was to the therapeutic value of A. aspera extract as a natural antioxidant in zebrafish. In this study, the H2O2 lethal dose (LD50) and A. aspera extract lethal concentration (LC50) on Zebrafish were determined. The results showed that the LD50 of H2O2 was 0.639 mM and A. aspera extract LC50 was 1110 μg/ml. Then, zebrafish adults were exposed to 0.639 mM H2O2 and treated with 1110 μg/ml A. aspera extract by coinduction of both on Zebrafish for 24 hours. Malondialdehyde (MDA) assay was performed to determine lipid peroxidation that occurred in the zebrafish. It was revealed that the A. aspera extract-treated group had substantially lower MDA levels than the negative control and normal groups, indicating a reduction in lipid peroxidation. The aqueous extract of A. aspera outperformed ascorbic acid as an antioxidant and also had beneficial therapeutic effects on the heart and brain of zebrafish.

Keywords: Achyranthes aspera L., antioxidant, hydrogen peroxide, MDA assay