Antioxidant efficacy of different formulae utilized in the yakrut roga to Non–Alcoholic Fatty Liver Disease
Piumi Dananga Chamari Perera, Hemakanthi Kulathunga, Liyanage Dona Ashanthi Menuka Arawwawala


Abstract

Context:
Liver diseases, particularly Non-Alcoholic Fatty Liver Disease (NAFLD),represent a significant worldwide public health challenge due to their prevalence and severe complications. It represents a spectrum of liver conditions ranging from simple steatosis to non-alcoholic steatohepatitis (NASH), leading to the development of liver fibrosis (scarring) and cirrhosis (irreversible scarring) as the final stage. Oxidative stress plays a crucial role in NAFLD pathogenesis, prompting the exploration of antioxidant therapy as a potential treatment avenue 
 
Objectives: This study explores the antioxidant potential of three Ayurvedic formulations phalatrikadi kwatha, arogyavardhana vati, and triphala guggulu in the context of NAFLD management. Methods: Methods involved meticulous preparation of aqueous extracts followed by in vitro assays to quantify Total Polyphenolic Content (TPC), Total Flavonoid Content (TFC), as well as antioxidant activities using 1,1-diphenyl-2-picryl hydrazyl (DPPH) and 2,2-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) radical scavenging activities, and Oxygen Radical Absorbance Capacity (ORAC) assays. 
 
Results: Results indicate substantial antioxidant activity in all three formulations, with Phalatrikadi kwatha exhibiting the highest values across assays. Notably,these formulations contain potent bioactive compounds such as phenolics and flavonoids, known for their hepatoprotective and anti-inflammatory properties. 
 
Conclusion: The study underscores the therapeutic potential of these Ayurvedic preparations in addressing NAFLD and other health conditions. Further research is recommended to explore additional extracts and concentrations, enhancing the understanding of their efficacy and expanding their therapeutic applications in contemporary healthcare. These findings highlight the relevance of Ayurveda medicine in modern healthcare paradigms and emphasize the importance of continued research in herbal and mineral preparations for human health and disease management.

Keywords: Antioxidant therapy, Ayurveda, Herbal preparations, NAFLD, Oxidative stress