Biological study and quality assessment of the oil obtained from seeds of Cannabis sativa L.
Ritika Shakya, Mijala Bajracharya, Bechan Raut
Abstract:
Context: Cannabis sativa L. (Hemp) seeds have been widely acknowledged by various ethnic groups and traditional healers. Objectives: phytoconstituents and quality assessment of the oil. Methods: The oil of hemp seed was extracted by Soxhletion method using n-hexane as the solvent. The quality of oil was analyzed for specific gravity, refractive index, saponification value, iodine value, acid value, peroxide value, and oil color index along with oil pigment determination. GC-MS, TPC, TFC, antioxidant, antimicrobial activity were evaluated. Results: The iodine value, saponification value, acid value, peroxide value were calculated to be 133.217 g/100g of oil, 203.467 mg KOH/g of oil, 2.197 mg KOH/g of oil and 3.815 mEq/kg respectively. GC-MS analysis identified 14 compounds. The oil sample showed Total Phenolic Content of 131.347 mg GAE/g and Total Flavonoid Content of 38.786 mg QE/g. The percent free radical scavenging assay for hemp seed oil a IC₅₀ value of 8.162μg/ml. The hemp seed oil demonstrated no antibacterial activity against E. coli and S. aureus. Conclusion: The hemp seed oil showed the presence of phytoconstituent with pharmacological activity that validates the therapeutic potential of Cannabis sativa L.
Keywords: antibacterial, Cannabis sativa L., GC-MS
Ritika Shakya, Mijala Bajracharya, Bechan Raut
Abstract:
Context: Cannabis sativa L. (Hemp) seeds have been widely acknowledged by various ethnic groups and traditional healers. Objectives: phytoconstituents and quality assessment of the oil. Methods: The oil of hemp seed was extracted by Soxhletion method using n-hexane as the solvent. The quality of oil was analyzed for specific gravity, refractive index, saponification value, iodine value, acid value, peroxide value, and oil color index along with oil pigment determination. GC-MS, TPC, TFC, antioxidant, antimicrobial activity were evaluated. Results: The iodine value, saponification value, acid value, peroxide value were calculated to be 133.217 g/100g of oil, 203.467 mg KOH/g of oil, 2.197 mg KOH/g of oil and 3.815 mEq/kg respectively. GC-MS analysis identified 14 compounds. The oil sample showed Total Phenolic Content of 131.347 mg GAE/g and Total Flavonoid Content of 38.786 mg QE/g. The percent free radical scavenging assay for hemp seed oil a IC₅₀ value of 8.162μg/ml. The hemp seed oil demonstrated no antibacterial activity against E. coli and S. aureus. Conclusion: The hemp seed oil showed the presence of phytoconstituent with pharmacological activity that validates the therapeutic potential of Cannabis sativa L.
Keywords: antibacterial, Cannabis sativa L., GC-MS
