Quantification of selected corticosteroids in herbal products
Nor Hayati Abdullah, Norulaiman Yusoff, Nurhazwani Mohd. Hirmizi
Abstract:
Context: Corticosteroids are a group of synthetic drugs that are very close in structure to cortisol. Objective: In this study, our focus is to optimize the HPLC method for several corticosteroids that might be added up in consumers' products, including tablets and capsules illegally. Methods: The corticosteroids were well separated by using the HPLC method of the gradient solvent system, in which the mobile phase consisted of acetonitrile, water, and methanol at a 1 mL/min flow rate and a column temperature of 40°C. The optimized HPLC method was validated based on the LOD, LOQ, linearity, recovery, repeatability, and precision in both product matrices. The parameter of extraction efficiency and matrix effect was also investigated. Results: The matrix effect investigation on different products of tablets and capsules showed that the matrix did not affect the analysis. All studied corticosteroids showed linearity in the range of 2-24 mg/mL with the R² value between 0.97 and 0.99. The recovery was between 88.20 and 119.50%, with LOD 0.37-0.73 mg/mL and LOQ 0.89-1.74 mg/mL. The intermediate precision for both product matrices was satisfied. The second extraction in the extraction efficiency investigation gave less than 10% from the first extraction. Conclusion: The developed HPLC test method could be used to analyze the presence and quantity of the studied corticosteroids. The sample preparation was optimized successfully, and it was indicated by the good recovery value.
Keywords: corticosteroids, HPLC, optimization, validation, herbal product
Nor Hayati Abdullah, Norulaiman Yusoff, Nurhazwani Mohd. Hirmizi
Abstract:
Context: Corticosteroids are a group of synthetic drugs that are very close in structure to cortisol. Objective: In this study, our focus is to optimize the HPLC method for several corticosteroids that might be added up in consumers' products, including tablets and capsules illegally. Methods: The corticosteroids were well separated by using the HPLC method of the gradient solvent system, in which the mobile phase consisted of acetonitrile, water, and methanol at a 1 mL/min flow rate and a column temperature of 40°C. The optimized HPLC method was validated based on the LOD, LOQ, linearity, recovery, repeatability, and precision in both product matrices. The parameter of extraction efficiency and matrix effect was also investigated. Results: The matrix effect investigation on different products of tablets and capsules showed that the matrix did not affect the analysis. All studied corticosteroids showed linearity in the range of 2-24 mg/mL with the R² value between 0.97 and 0.99. The recovery was between 88.20 and 119.50%, with LOD 0.37-0.73 mg/mL and LOQ 0.89-1.74 mg/mL. The intermediate precision for both product matrices was satisfied. The second extraction in the extraction efficiency investigation gave less than 10% from the first extraction. Conclusion: The developed HPLC test method could be used to analyze the presence and quantity of the studied corticosteroids. The sample preparation was optimized successfully, and it was indicated by the good recovery value.
Keywords: corticosteroids, HPLC, optimization, validation, herbal product
