Enchancing tissue culture efficiency and growth of Bryophyllum pinnatum (Lam.) Oken with hydrogen peroxide treatment
Vansak He, Lina Ly, Sophearom Chhea, Sokunvary Oeung, Huysean Huot, Sin Chea,Visessakseth So
Abstract:
Context: Bryophyllum pinnatum (Lam.) Oken is a well-known medicinal plant in Cambodia, traditionally used for treating various health conditions. In vitro tissue culture of this species faces significant challenges from microbial contamination, especially during surface sterilization. Objective: to compare the effectiveness of hydrogen peroxide (H₂O₂) and sodium hypochlorite (NaOCl) in reducing contamination and promoting explant growth on Murashige and Skoog (MS) medium. Methods: fresh B. pinnatum leaves were sterilized by using either 5.5% H₂O₂ for 20 mins or 3% NaOCl for 30 mins. Sterile explants were cultured on Murasige and Skoog medium under aseptic conditions. Contamination and survival rated were record and growth parameters were measure four weeks. Results: 5.5% H₂O₂ (20 min) reduced contamination to 58% and supported 14% survival of explants, which developed plantlets reaching 3.4 cm by week 4. In contrast, NaOCl resulted in 100% contamination and no viable explants. Conclusion: H₂O₂ treatment is a practical and effective method for improving the micropropagation of B. pinnatum.
Keywords: Murashige medium, medicinal plant, Skoog medium, surface sterilization
Vansak He, Lina Ly, Sophearom Chhea, Sokunvary Oeung, Huysean Huot, Sin Chea,Visessakseth So
Abstract:
Context: Bryophyllum pinnatum (Lam.) Oken is a well-known medicinal plant in Cambodia, traditionally used for treating various health conditions. In vitro tissue culture of this species faces significant challenges from microbial contamination, especially during surface sterilization. Objective: to compare the effectiveness of hydrogen peroxide (H₂O₂) and sodium hypochlorite (NaOCl) in reducing contamination and promoting explant growth on Murashige and Skoog (MS) medium. Methods: fresh B. pinnatum leaves were sterilized by using either 5.5% H₂O₂ for 20 mins or 3% NaOCl for 30 mins. Sterile explants were cultured on Murasige and Skoog medium under aseptic conditions. Contamination and survival rated were record and growth parameters were measure four weeks. Results: 5.5% H₂O₂ (20 min) reduced contamination to 58% and supported 14% survival of explants, which developed plantlets reaching 3.4 cm by week 4. In contrast, NaOCl resulted in 100% contamination and no viable explants. Conclusion: H₂O₂ treatment is a practical and effective method for improving the micropropagation of B. pinnatum.
Keywords: Murashige medium, medicinal plant, Skoog medium, surface sterilization
