Antithrombotic and antioxidant effects of Sophora japonica flower buds collected in different provinces in Vietnam, in correlation with rutin and other phytochemicals content
Viet Duy Anh Nguyen, Thi Van Anh Nguyen, Ngoc Duy Pham, Thu Hien Duong, Hong Luyen Le
Abstract:
Context: flower buds of Sophora japonica L. (SJ), a great source of rutin, have been popular for several pharmacological effects, but limited studies were concerned with the correlation of rutin and the bioactivities of this plant. Objective: to evaluate the ex vivo antithrombotic and in vitro antioxidant activities of rutin and SJ extracts. Methods: the antiaggregatory and anticoagulant activities were investigated on human plasma. The antioxidative effect was determined by different radical scavenging assays. Rutin content, total phenolic content, and flavonoid content were analyzed and correlated with the bioactivities. The results showed that most SJ samples exhibited platelet aggregation inhibitory effects by reducing the maximal aggregation percentage and lowering the aggregation speed. Moreover, these samples were able to prolong the blood coagulation time via the intrinsic and common pathways. Rutin showed a weaker antithrombotic effect than SJ samples, suggesting that components in the plant extracts might possess a synergic inhibitory action on blood clot formation. All samples expressed a strong scavenging ability against both DPPH and ABTS radicals (with IC50 lower than 50 ɥg/mL), but a moderate effect on reducing H2O2. Pearson correlation analysis revealed that rutin, TFC, and TPC were strongly correlated with the antithrombotic effect of the plant extracts. Meanwhile, rutin content and TFC significantly correlated with H2O2 assay while TPC was remarkably associated with DPPH and ABTS assays. Conclusion: the findings in this study provide a reference for exploring S. japonica samples in the prevention and treatment of thrombosis- and oxidative stress-related diseases.
Keywords: antioxidant, antithrombotic, phytochemical-activity correlation rutin, Sophora japonica
Viet Duy Anh Nguyen, Thi Van Anh Nguyen, Ngoc Duy Pham, Thu Hien Duong, Hong Luyen Le
Abstract:
Context: flower buds of Sophora japonica L. (SJ), a great source of rutin, have been popular for several pharmacological effects, but limited studies were concerned with the correlation of rutin and the bioactivities of this plant. Objective: to evaluate the ex vivo antithrombotic and in vitro antioxidant activities of rutin and SJ extracts. Methods: the antiaggregatory and anticoagulant activities were investigated on human plasma. The antioxidative effect was determined by different radical scavenging assays. Rutin content, total phenolic content, and flavonoid content were analyzed and correlated with the bioactivities. The results showed that most SJ samples exhibited platelet aggregation inhibitory effects by reducing the maximal aggregation percentage and lowering the aggregation speed. Moreover, these samples were able to prolong the blood coagulation time via the intrinsic and common pathways. Rutin showed a weaker antithrombotic effect than SJ samples, suggesting that components in the plant extracts might possess a synergic inhibitory action on blood clot formation. All samples expressed a strong scavenging ability against both DPPH and ABTS radicals (with IC50 lower than 50 ɥg/mL), but a moderate effect on reducing H2O2. Pearson correlation analysis revealed that rutin, TFC, and TPC were strongly correlated with the antithrombotic effect of the plant extracts. Meanwhile, rutin content and TFC significantly correlated with H2O2 assay while TPC was remarkably associated with DPPH and ABTS assays. Conclusion: the findings in this study provide a reference for exploring S. japonica samples in the prevention and treatment of thrombosis- and oxidative stress-related diseases.
Keywords: antioxidant, antithrombotic, phytochemical-activity correlation rutin, Sophora japonica
